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Lecturer(s)
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Váňová Ivana, MVDr. Ph.D.
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Doležalová Jana, MVDr. Ph.D.
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Řeháková Kristína, MVDr. Ph.D.
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Matalová Eva, prof. RNDr. Ph.D.
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Váňová Ivana, MVDr. Ph.D.
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Course content
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Lectures: 1. Principles of basic laboratory methods 2. Criteria of laboratory tests, reference intervals, species/breed differences 3. Good laboratory practice, quality management, indications of laboratory tests, laboratory profiles and algorithms 4. Pre-analytical and analytical phase of laboratory test - major mistakes 5. Lab. diagnostics of blood and haematopoietic organ disorders 6. Lab. diagnostics of cardiovascular and respiratory system disorders 7. Lab. diagnostics of gastrointestinal tract disorders 8. Lab. diagnostics of liver disorders 9. Lab. diagnostics of uropoietic system disorders 10. Lab. diagnostics of endocrinopathies 11. Lab. diagnostics of the immune system and skin disorders 12. Lab. diagnostics of nervous system and musculoskeletal system disorders 13. Consults, results of the credit test Practical lessons: 1. Patient preparation, sample collection, processing and storage (blood, urine, effusion, CSF, SF, BAL) 2. Haematological examination I 3. Haematological examination II 4. Cytological examination 5. Urine analysis 6. Laboratory examination of effusions 7. Credit proceeding: test
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Learning activities and teaching methods
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Lectures, Seminars, Laboratory and desk-based work
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Learning outcomes
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The subject is focused on diagnostic tools used in small animal laboratory medicine. Practicals are based on complex laboratory analyses (biochemistry, haematology, cytology) of routinely available biological samples (blood, urine, effusion and others). Practicals include presentations of clinical cases representing basic pathologies diagnosed based on laboratory methods. Results from cytological evaluation of pathological samples from patients are presented as protocols focused on the description of microscopic findings and their interpretation.
1. Student is able to explain the principles of basic laboratory methods. 2. Student knows the necessary conditions for sample collection, processing and storage/transport for routine biological samples and understands the difficulties of the pre-analytical and analytical phases of laboratory analysis and is aware of test results limitations. 3. Student is able to indicate appropriate diagnostic tests and to interpret basic laboratory abnormalities observed in the most common organ disorders. 4. Student is able to prepare and evaluate blood smears, to perform complete urine analysis and to process effusions for cytological evaluation. 5. Student is competent to perform basic laboratory tests of routinely collected body fluids and based on the results interpret basic pathologies.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written credit test,
Credit conditions: 1. To fulfil the requirements (written tests, continual verification of the knowledge in oral form, final credit test) 2. To prepare protocols from cytological evaluation
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Recommended literature
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Latimer, K. S. Mahaffey, E. A., Prasse, K. W. Duncan and Prasse's veterinary laboratory medicine. Clinical Pathology.. Blackwell, 2003.
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Raskin R., Meyer D. Canine and feline cytology.. Saunders, 2015.
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Stockham, S. L., Scott, M. A. Fundamentals of veterinary clinical patology.. Blackwell, 2002.
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Tvedten H., Willard M. Small animal clinical diagnosis by laboratory methods. Saunders, 2012.
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Valenciano A. C., Cowell R. L. et al. Diagnostic cytology and haematology of the dog and cat. Mosby, 2014.
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