Course: Laboratory Diagnostics of Farm Animals

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Course title Laboratory Diagnostics of Farm Animals
Course code 1680/VALDP
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 2
Language of instruction English
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Course availability The course is available to visiting students
Lecturer(s)
  • Illek Josef, doc. MVDr. DrSc.
  • Čech Svatopluk, doc. MVDr. Ph.D.
  • Šimková Ivana, MVDr.
  • Tulis František, MVDr.
  • Gricová Jana, MVDr. Ph.D.
  • Bezděková Zdenka, MVDr.
  • Kadek Romana, MVDr. Ph.D.
  • Staffa Andrea, Mgr. Ph.D.
Course content
Lectures: Introduction to the discipline, importance of laboratory examinations in individual and group diagnosis of diseases, disorders of metabolism and production. General principles hematological examination, diagnostic significance of basic hematological parameters. Colostral immunity, the importance of individual components of colostrum and diagnosis of colostral immunity. Laboratory diagnosis of rumen fermentation disorders and indigestion diagnostics. Laboratory diagnosis of ABR disorders, energy, protein and mineral metabolism. Diagnosis of vitamins deficiency. Laboratory diagnosis of organ diseases and enzymodiagnostics. Laboratory diagnosis of respiratory and diarrheal diseases of ruminants and pigs. Laboratory diagnosis of mastitis. Practical exercises: Hematological examination - appropriate sampling material, principles of sampling, storage and preservation of samples. Determination of hematocrit and complete blood count by an automatic analyzer, preparation and staining of blood smear, determination leukogram. Interpretation of test results. Laboratory examination of colostral immunity - appropriate sampling material, principles of sampling, storage and preservation of samples. Refractometric determination of total protein in blood serum, immunoglobulins in colostrum and blood serum, GMT, vit. A and E in blood serum and colostrum quality. Interpretation of test results. Laboratory examination of rumen fluid - appropriate sampling material, principles of sampling, storage and preservation of samples. Determination of pH, titratable acidity, volatile fatty acids, lactic acid and the number of ciliates. Interpretation of test results. Biochemical examination and examination of mineral elements - appropriate sampling material, principles of sampling, storage and preservation of samples. Determination of acid-base balance and basic analytes in blood, blood serum, urine, milk. Interpretation of test results. Bacteriological examinations of the upper respiratory tract secretions, bronchoalveolar lavage and feces. Detection of viral pathogens of respiratory and diarrheal diseases of calves. Bacteriological examinations of milk, detection of major mastitis pathogens. Interpretation of test results, anamnesis, diagnosis of the disease, suggesting appropriate treatment. Credit test.

Learning activities and teaching methods
Lectures, Extramural practical training, External Practical Training
Learning outcomes
The aim of the course is to teach laboratory methods used to diagnose diseases of food animals. Graduates will learn practically laboratory procedures suitable for diagnostics of selected nosological units.
Theoretical knowledge The student can describe and explain the principles of laboratory analysis of biological materials. Practical skills The student can take samples of biological materials and perform basic laboratory analyses. Competence The student can collect, store and transport samples, choose appropriate diagnostic tests, interpret the results and use them in practice.
Prerequisites
unspecified

Assessment methods and criteria
Oral exam, Written exam, Written credit test

Participation in practical exercises, credit test.
Recommended literature
  • Kaneko J.J. Clinical Biochemistry of Domestic Animals. Academic Pres. INC San. Diego California, 2008. ISBN 9780123704917.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester