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Lecturer(s)
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Celer Vladimír, prof. MVDr. Ph.D.
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Molinková Dobromila, MVDr. Ph.D.
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Salát Jiří, RNDr. Ph.D.
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Course content
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Lectures 1. Basic characteristics of the immune system and its functions 2. Cells and molecules of the immune system 3. Cells and molecules involved in non-specific immunity and their functions 4. Complement and inflammation 5. Lymphocytes and specific immunity 6. Antigen presentation and regulation at the level of Th lymphocytes 7. Cytotoxic lymphocytes and antibodies, effector mechanisms of specific immunity Practicals 1. Serology I - agglutination 2. Serology II - precipitation 3. Serology III - ELISA 4. Leukocyte count tests, phagocytosis tests 5. Serology IV - complement fixation test 6. Tests of lymphocyte activity 7. Antibodies, detection of their total levels in serum
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Learning activities and teaching methods
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Lectures, Seminars, Laboratory and desk-based work
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Learning outcomes
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The aim of the subject is to provide basic information on the structure and function of the immune system, including the specifics in different farm and domestic animal species and categories, its importance in the defence against infectious and tumour diseases, causes and mechanisms of the development of the immune system disorders and basic applications under practical conditions. In the lectures, mechanisms of nonspecific and specific immunity, defence mechanisms in infectious and non-infectious processes and the causes and mechanisms of the immunopathological conditions will be explained. Practicals, which are carried out in a form of practical training, deal with fundamentals of serology and methods of clinical immunology and vaccinology.
Acquired competencies = learning outcomes 1. The student is able to name and characterize the individual components of the immune system (organs, cells, molecules) 2. The student is able to describe and characterize all important mechanisms of non-specific and specific (molecular and cellular) immune response 3. The student knows the methods used in veterinary laboratory practice and can practically perform basic tasks associated with serological methods - working with serum samples, their titration, sample incubation, evaluation and interpretation of results obtained on individual instruments (ELISA reader, spectrophotometer) 4. The student knows the main methods of monitoring the number and activity of cells of the immune system, including working with cells, isolation of individual cell populations, performing individual tests, observation of cells in optical and fluorescence microscopy and how to interpret the results.
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Prerequisites
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The course builds on previous courses, especially histology, physiology, biochemistry and microbiology
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Assessment methods and criteria
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Written credit test
Conditions for obtaining credit: attendance and active participation in all exercises, credit test
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Recommended literature
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Day, M.J., Schultz, R.D. Veterinary immunology - principle and practice. 2014.
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Tizard. Veterinary Immunology.
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Toman, M. a kol. Practical skills in immunology. VÚVeL Brno, 2009.
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Toman. Veterinární imunologie. Praha, 2000.
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